Using trypsin digestion or cell scraper to separate adherent cells will affect the expression of cell surface proteins, damage cell health and reduce cell viability.
Temperature-Responsive cell culture surface is coated by an even and unique nano-polymer. When the temperature of the culture is reduced from 37℃to4℃, the temperature-responsive surface gradually changes from slightly hydrofobic to hydrophilic, resulting in adherent cells harvest without trypsin involved. By using this gently method, avoid cell damaging by scraper or trysin to preserve high viability and intact surface receptors and antigens of cells, to realize cell harvest without damage and continuous subculture procedure.
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