After the surface treatment of the target plate, the protein binding capacity is greatly enhanced, which can reach 300-400ng igG/cm², and the molecular weight of the main bound protein is >10kD. The use of this type of ELISA plate can improve sensitivity, and can relatively reduce the concentration and dosage of coating protein. The disadvantage is that it produces non-specific reactions, antigen or resistance reactions. After antigen or antigen coating, non-ionic detergent can not effectively seal the unbound protein site, and protein should be used as a sealant.







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