Oropharyngeal Swab

Flocked Swabs are ideal for collecting large amount of cells and rapid solution of the specimens instantly release the cells into the cells into the transport medium. Flocked swabs utilize innovative nylon fibre technology to provide outstanding sample collection and release. Nylon fiber swab that allows a better clinical sample collection and the following immediate release into the liquid transport medium. It has a plastic shaft with"breakpoint" to allow the swab break into the test tube and successive collection by screw"capture" cap after the tube closing.

The first step of nucleic acid detection is sample preparation, i.e. nucleic acid extraction, which is completed in a specific safety cabinet.

A novel coronavirus suspected case of nasopharyngeal swabs was installed in the outer spiral tube of 15 ml. In order to prevent the sampling tube from happening suddenly, Lei Xiaonian wrapped three layers of plastic bags in the outer spiral tube and placed them on the vortex oscillator to perform the whirlpool oscillation, so as to mix the samples well.

This is a critical and most dangerous step, because in this process, the samples will produce aerosols, which will easily cause occupational exposure if the protection is not in place. Because of this, Lei Xiaonian's operation is more cautious.

After the sample is mixed evenly, it will be added into the automatic nucleic acid extraction kit, and then it will be allowed to stand for a period of time, so that the sample and reagent can fully function, crack the virus, and then extract the virus nucleic acid with the nucleic acid extractor.

When this step is over, it means that the most dangerous nucleic acid extraction is over.

To prevent secondary pollution, two other colleagues will disinfect the test area. At this time, Lei Xiaonian also took off the three-level protective equipment, replaced the two-level protective equipment, started to configure the nucleic acid detection reaction system, and put the configured reaction system into the real-time fluorescent PCR instrument for nucleic acid amplification reaction detection.

After about 3 hours, the test results will come out. For the positive samples, they will replace the test reagent, carry out repeated secondary tests, and further verify the test results.

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