Color and purpose of blood collection tube
Blood collection tubes are usually divided into red, orange, golden, green, light green, purple,9 colors of light blue, black and gray.
details as follows:
1. (Ordinary serum tube)- Red head cover:
The blood collection tube contains no additives and is suitable for routine biochemical serum tests, blood bank and serological related tests;
2. (Quick Serum Tube)-Orange red head cover: There is a coagulant in the blood collection tube, which can activate fibrinase and turn soluble fibrin into insoluble fibrin polymer, and then form a stable fibrin clot. The rapid serum tube can coagulate the collected blood within 5 minutes, which is suitable for serialized emergency serum test.
3. (Inert Separation Gel Coagulation Accelerator Tube)-Golden head cap: Inert separation gel and coagulant are added to the blood collection tube. After the specimen is centrifuged, the inert separating gel can completely separate the liquid components (serum or plasma) and solid components (red blood cells, white blood cells, platelets, fibrin, etc.) in the blood and completely accumulate in the center of the test tube to form a barrier. The specimen is within 48 hours keep it steady. A coagulant can quickly activate the coagulation mechanism and accelerate the coagulation process, which is suitable for emergency serum biochemical tests;
4. (Heparin anticoagulation tube)-green head cover: heparin is added to the blood collection tube. Heparin directly has the effect of antithrombin, which can prolong the clotting time of the specimen. It is suitable for red blood cell fragility test, blood gas analysis, hematocrit test, erythrocyte sedimentation rate and general energy biochemical determination, not suitable for blood coagulation test. Excessive heparin can cause the accumulation of white blood cells and cannot be used for white blood cell counting. It is not suitable for white blood cell classification because it can stain the blood slice with a light blue background.
5. (Plasma separation tube)-light green head cover: Adding lithium heparin anticoagulant into the inert separation tube can achieve the purpose of rapid plasma separation. It is the best choice for electrolyte testing, and can also be used for routine plasma biochemical determination and ICU Wait for emergency plasma biochemical testing. Plasma samples can be directly put on the machine and kept stable for 48 hours under refrigeration.
6. (EDTA anticoagulation tube)-purple head cover: ethylenediaminetetraacetic acid (EDTA, molecular weight 292) and its salt are an amino polycarboxylic acid, which can effectively chelate calcium ions in blood samples, chelate calcium or Removal of the calcium reaction site will block and terminate the endogenous or exogenous coagulation process, thereby preventing the blood specimen from clotting. Suitable for general hematology tests, not suitable for coagulation tests and platelet function tests, and not suitable for the determination of calcium ion, potassium ion, sodium ion, iron ion, alkaline phosphatase, creatine kinase and leucine aminopeptidase And PCR test.
7. (Sodium citrate coagulation test tube)-light blue head cover: Sodium citrate mainly acts as an anticoagulant by chelating with calcium ions in the blood sample. It is suitable for blood coagulation experiments. The recommended concentration of anticoagulant recommended by the National Committee for Laboratory Standardization (national committee for clinical laboratory standards, NCCLS) is 3.2% or 3.8% (equivalent to 0.109mol/L or 0.129mol/L), anticoagulant The ratio of agent to blood is 1:9.
8. (Sodium citrate erythrocyte sedimentation rate test tube)-black head cover: The sodium citrate concentration required for erythrocyte sedimentation rate test is 3.2% (equivalent to 0.109mol/L), the ratio of anticoagulant to blood is 1:4. Used for erythrocyte sedimentation rate (ESR) detection.
9. Blood sugar tube, gray: the blood collection tube contains sodium fluoride, which is a weak anticoagulant, and is usually used in combination with potassium oxalate or sodium diiodate. The ratio is 1 part of sodium fluoride and potassium oxalate. 3 copies. 4mg of this mixture can prevent 1ml of blood from coagulating and inhibit sugar decomposition within 23 days. It is a good preservative for blood glucose determination. It cannot be used for the determination of urea by the urease method, nor is it used for the determination of alkaline phosphatase and amylase. Recommended for blood glucose testing.
