Colored PCR Plate Can Be Used As Sterile DNA RNA FREE

Color PCR (Color complement assay) literally translates as "color complementation detection", which is a kind of LP-PCR. Color PCR has a clearer translation: it uses a fluorescent dye to label the 5'end of the primer. The fluorescent dyes JOE and FAM are green fluorescence; TAMRA is red fluorescence; COUM is blue fluorescence. Different fluorescently labeled primers participate in the reaction at the same time, and the amplified target gene will have the dye at the 5'end of the primer. Through electrophoresis or centrifugal precipitation, the naked eye can judge whether the target gene exists and the type of amplified gene according to the color of different fluorescence. . Usually only two primers of different colors are needed, one is used as a gene detection primer; the other is used as an internal control to control the conditions to diagnose gene deletion, chromosomal translocation or infection with a certain virus. When detecting multiple point mutations, more colors can be used. For example, genetic diseases with multiple point mutations, several suspected viral infections, and HLA site analysis can use color PCR to detect multiple sites at the same time.