The 12-channel pipette is a single-channel micropipette commonly used in biological and chemical laboratories for small-volume transfer. The pipette has the characteristics that it can be calibrated by the user without the aid of tools, and is easy to disassemble and maintain. At the same time, it is fully ergonomic. The pipette has five modes: pipetting mode, sample mixing mode, reverse aspiration mode, electrophoresis loading mode, and graded pipetting mode.
12-channel pipette pipetting method:
Before pipetting, make sure that the pipette, tip and liquid are at the same temperature. When aspirating the liquid, keep the pipette upright and insert the pipette tip 2-3 mm below the liquid surface. Wet the nozzle by aspirating the liquid a few times before suctioning (especially if you are picking up liquids that are viscous or have a different density than water). There are two pipetting methods available at this time.
is the forward pipetting method. Press the button with your thumb to the stop, then slowly release the button to return to the origin. Then press the button to the stop point to discharge the liquid, stop for a while and continue to press the button to the second stop point to blow out the remaining liquid. and then release the button.
The second is the reverse pipetting method. This method is generally used to transfer high-viscosity liquids, biologically active liquids, easily foaming liquids or trace amounts of liquids.
First press the button to the second stop, and slowly release the button to the origin. Then press the button to the stop point to discharge the liquid with the set range, continue to hold the button at the stop point (don't press it down again), remove the pipette tip with residual liquid, and discard it.
Let's take a look at five common fault causes and solutions in the use of 12-channel pipettes:
1. There is residual liquid in the suction nozzle of the 12-channel pipette:
This situation occurs because the suction nozzle is not suitable, and the original suction nozzle of the pipette should be replaced immediately.
2. The pipette is blocked and the suction volume is too small:
This happens because the liquid has penetrated into the pipette. Clean and lubricate the piston and nozzle connection.
3. Beyond the limited scope:
This happens because the pipette is damaged and should be sent to a repair point for repair.
Fourth, the ejector of the pipette suction nozzle is stuck or does not work properly:
This situation occurs due to the contamination of the suction nozzle connection. The ejector sleeve should be removed and cleaned with 75% ethanol.
5. The pipette leaks or the pipetting volume is too small: There are several reasons for this situation:
1. The piston and O-ring are not sufficiently lubricated.
2. The pipette is contaminated.
3. There is a foreign body between the suction nozzle and the connecting piece.
4. The suction nozzle is not suitable.
5. Improper installation of suction nozzle.
6. The plastic of the suction nozzle is not uniformly wet.
When the 12-channel pipette has a pipetting failure, you should:
1. Add appropriate amount of silicone oil
2. Clean and lubricate the piston and suction nozzle connection
3. Clean the suction nozzle connection and install a new suction nozzle
4. Use the original suction nozzle
5. Re-tighten
6. Install a new suction nozzle.
