Pipettes and tips are a complete system. Only the same manufacturer can guarantee the accuracy and precision of the results. When calibrating and using pipettes on a daily basis, matching tips from the same manufacturer must be selected. To mount the tip using the micro-rotation mounting method, insert the pipette tip vertically into the tip, gently rotate and tighten. It is forbidden to knock up and down or shake left and right to avoid deformation of the suction head and wear of the suction nozzle under strong friction. Some micropipettes have very thin tips, and hard tapping may damage the tips.
Checking the tightness of the pipette tip pipette
Whether the tightness of the pipette is normal or not directly affects the accuracy of the results. After making sure that the pipette is using a support tip from the same manufacturer and tightening the tip, a simple visual inspection method can be used to check the airtightness of the pipette. ul Immerse the tip of the following pipette by 1-2) mm, wait for 20s, and observe whether the liquid level in the tip drops. If it drops, it means that the pipette is leaking. For pipettes above 1000ul, place them vertically for 20s after aspiration, and observe whether there is liquid dripping from the tip of the tip. If there is liquid leakage from the pipette.
The types of pipettes are becoming more and more diverse. Experimenters should choose appropriate calibration points and electronic balance standards according to different types of pipettes, use pipette tips from the same manufacturer and correct pipetting operations, and regularly check the pipettes. The capacity is calibrated to ensure the accuracy and consistency of its capacity. When the indicated value of the pipette is out of tolerance, first check whether the tightness of the pipette is normal, and then judge whether it can be calibrated in the laboratory.
