Introduction to PCR consumables
The high-throughput 96-well PCR plate and 8-strip tube are mainly used as primers, Taq DNA polymerase, dNTPs, template nucleic acids, Mg, buffers involved in the amplification reaction in the polymerase chain reaction (PCR). liquid carrier.
pcr plate
Because of its purity and stability, medical-grade polypropylene (PP) material can better adapt to repeated high and low temperature settings in the PCR reaction process, and can achieve high temperature and high pressure sterilization operations, and is often used in the production of PCR consumables.
Different volumes of PCR tubes/plates, how do I choose?
Choose the appropriate product according to the specific experimental requirements. The volume of PCR tubes can mostly meet the requirements of PCR reactions. On this basis, it is recommended that low-capacity tubes be preferred. Because the low volume reactor tubes/plates have less overhead space, thermal conductivity is improved and evaporation is reduced. At the same time, it is necessary to avoid adding too much or too little when adding samples. Too much can lead to reduced thermal conductivity, spillage and cross-contamination, while too little can cause evaporative loss of the sample.
How to properly use 96-well PCR plate?
1. The PCR plate is available in transparent and milky white, among which the milky white is more suitable for the new fluorescence quantitative PCR instrument that collects fluorescent signals on the top. But if the real-time PCR instrument is bottom light source, it should choose transparent.
2. The PCR plate can be divided into three design modes: no skirt, half skirt and full skirt according to the design of the skirt. PCR plate.
3. The PCR plate is divided into 100ul volume (low plate) and 200ul volume (high plate), according to different models, choose different height plates to avoid the damage to the machine caused by the height error and the data distortion caused by uneven heating.
4. When using a 96-well plate for real-time PCR experiments, it is recommended to use an optical film to seal the reaction wells. The correct method of sealing is: first along the longitudinal direction of the 96-well plate, then transversely, and finally along the edge of the plate to seal.
pcr plate
Yongyue's PCR plate product series includes 96 aperture, 384 aperture, and the capacity range is 100ul, 200ul, 40ul, 50ul. The skirt design has half skirt, no skirt PCR plate, high skirt and full skirt. Four design classifications; product colors are transparent, white, etc. to choose from.
