In the microbiology laboratory, an experimental equipment is often used, that is, a cryovial. However, due to their varying levels of sophistication, the effects vary widely. For this reason, most of the domestic laboratories currently make their own strain preservation tubes, which not only increases the work intensity, but also due to various conditions, the strain preservation effect is not always satisfactory. Therefore, it is necessary to master the use of cryopreservation tubes and some precautions, so as to play a great role.
1. How to use
1. When using cryovials to store samples, it is strictly required that the cryovials should be stored in the vapor layer of liquid nitrogen or in a refrigerator. If the cryovial is stored in liquid nitrogen liquid, there is a certain chance that liquid nitrogen will penetrate into the interior of the cryovial, and the liquid nitrogen gasification during resuscitation will cause the pressure inside and outside the tube to be unbalanced, which will easily cause the cryovial to burst, and is biohazardous. .
2. To operate the cryopreservation tube recovery, use safety protection equipment throughout the process. It is recommended to wear a laboratory coat, cotton gloves and operate on a safe laboratory bench. Wear goggles or a face shield if possible. Please be extra careful as the indoor temperature will be higher in summer than in winter.
3. During the storage of cryopreserved cells, the freezing temperature of the cryopreservation tube must be uniform. Uneven freezing will result in ice plugs that inhibit the transfer of liquid temperature on both sides and create dangerously high pressures that can lead to freezing
Storage is damaged.
4. The volume of frozen samples should not exceed the maximum working volume required by the cryovials.
2. Matters needing attention
1. Storage environment of cryovials Unused cryovials can be stored at room temperature or 2-8°C for 12 months; inoculated cryovials can be stored at -20°C and have good strain preservation effect within 12 months ; The inoculated cryopreservation tube can be stored at -80 ℃, and the strain can be preserved within 24 months. 2. Storage time of cryovials Unused cryovials can be stored at room temperature or 2-8°C; inoculated cryovials can be stored at -20°C or -80°C.
3. The steps of using the cryopreservation tube Pick a fresh culture from the pure bacterial culture and prepare it into a bacterial suspension with a McFarland turbidity of about 3-4. -5 times to emulsify the bacteria, do not shake; put the preservation tube in the refrigerator (-20℃--70℃)
The above is the introduction to the use of cryopreservation tubes and precautions. I hope everyone can learn to master these knowledge points, which will be very helpful for our follow-up operations.
