Tell you about the use and precautions of test tubes and centrifuge tubes. The graduated centrifuge tube is a measuring device, suitable for qualitative analysis of micro and semi-micro. When centrifuging and stratifying substances suspended in liquid, a graduated centrifuge tube is an indispensable tool. Next, I will explain it in detail for everyone, hoping to help everyone.
1. How to use the centrifuge tube 1. Because the graduated centrifuge tube is a measuring device, it must be cleaned and dried before use. 2. When reading, be sure to observe the meniscus correctly, otherwise certain errors will be introduced. 3. When using, the number of centrifuge tubes should be determined according to the model of the centrifuge. If a centrifuge with double cannula is used for a single test, the same volume of liquid must be placed in the other centrifuge tube to maintain balance. 4. The centrifuge tube and the centrifuge sleeve should be matched. The tube should not be too long, otherwise it is easy to break when turning. But it is not easy to be too short, too short tubes are unstable when rotating in the air, and are prone to breakage. If the sleeve is too thick, it will not enter, and if it is too thin, it will be unstable when rotating. The round mouth should be larger than the inner diameter of the sleeve. 5. After the centrifuge tube is put into the casing of the centrifuge for stratified separation, it should be stopped naturally when it stops rotating, and it must not be forced to stop by external force.
2. Precautions for centrifuge tubes When using centrifuge tubes, do not use one tube for multiple times. Pay attention to the volatilization of samples and the leakage of some radioactive or corrosive samples; during the storage process, it is necessary to seal them well; To prevent deformation of the centrifuge tube during use.
3. Test tube usage and precautions:
1. Ordinary test tubes can be directly heated, and the outer wall of the test tube should be wiped dry before heating
2. When filling the solution, it should not exceed 1/2 of the capacity of the test tube, and when heated, it should not exceed 1/3 of the test tube.
3. When heating, a test tube clamp must be used. The test tube clamp is inserted from the bottom of the test tube to the test tube mouth. When taking it, it is also taken out from the lower part of the test tube and clamped in the upper part of the test tube mouth (close to 1/3 of the test tube mouth).
4. When heating, first make the test tube evenly heated, then focus on heating at the bottom of the test tube, and keep moving the test tube. The test tube should be inclined at about 45°, and the mouth of the tube should not face yourself or others.
5. Pour the liquid into the test tube, you can only take one test tube at a time, but when comparing several test tubes, you can hold several in your hand at the same time (with your thumb and palm).
6. When shaking the test tube, hold the test tube with three fingers in the thumb and food, and use the wrist instead of swinging the arm to shake vigorously. The liquid in the test tube will not splash out due to centrifugal force.
7. The heated test tube cannot be put into the test tube rack immediately to prevent the test tube rack from being scalded.
8. The cleaning test tube can be directly shaken with water or brushed with the test tube. If necessary, it must be brushed with washing powder. According to the drugs used in the experiment, acid, alkali and acid potassium dichromate lotion can also be used for cleaning. After the test tube, there should be no water droplets hanging on the inner wall
Hangzhou Leyu Medical Equipment Co., Ltd. is led by a professor-level senior engineer, a Ph.D. and a R&D and sales team in the field of life sciences for more than ten years. strength. Mainly include: such as PCR tubes, centrifuge tubes, non-enzyme and non-pyrogenic diagnostic packaging bottles, non-enzyme and non-pyrogenic tips, cell culture, microtiter plates, detection plates, deep-well plates, magnetic sleeves, used for vaccines, antibody drugs, Gene therapy drugs, cell therapy drugs, protein drugs, nucleic acid drugs, drug development and nucleic acid extraction (DNA and RNA), gene sequencing, molecular diagnosis, vaccine research and development. Research in cytology, genetics, immunology, experimental medicine, biomolecular science and other disciplines
