For so-called precision laboratory consumables, the esoteric principles must be explained in detail most of the time, but the principle of the pipette is simple - the piston moves up and down by the retracting force of the spring to expel or suck the liquid.
Generally speaking, there are two types of pipettes, one is the exhaust type; the other is the external piston type, which is often used as a special pipette and has a relatively narrow scope of application. This type of pipette can be used to pipette high viscosity. sample.
The so-called exhaust type is to push down the piston to push out the air inside the lower end of the pipette, and then when the piston moves upward, the air pressure inside the lower end of the pipette is less than the external air pressure, so that it can suck up the liquid under the action of the external air pressure. . In short, air out, liquid in!
The so-called outer piston is actually exactly the same as the syringe. After reading the working process of the syringe, you can probably understand the principle of the outer piston.
Pipettes are laboratory instruments commonly used in biological and chemical laboratories to remove trace amounts of liquids. The advantages are simple operation and high precision.
The basic maintenance method of the pipette:
1. In order to prevent the pipette from being damaged, hang the pipette on a special bracket when not in use, and return to the maximum range after use on the same day;
2. For potentially corrosive liquids, use a pipette tip with a filter element, and select a pipette suitable for operation (exhaust type and external piston type);
3. Use the recommended grease on the lubricating seals and regularly clean the outside of the pipette/pipette tip with an isopropyl alcohol solution;
4. No calibration is required after routine maintenance, and it is only necessary to re-calibrate when replacing or repairing the piston.