As we all know, pipette tips are a common biomedical laboratory consumable.
1. Use suitable tips
To ensure better accuracy and precision, it is recommended that the pipetting volume be within the 35%-100% range of the tip.
2. Installation of the tip
For most brands of pipettes, especially multi-channel pipettes, it is not easy to install the tip: in order to pursue a good seal, it is necessary to insert the pipette handle into the tip and turn it left and right or back and forth to tighten it. . Some people also use the pipette to repeatedly hit the tip to tighten, but this operation will cause the tip to deform and affect the accuracy, and in severe cases, the pipette will be damaged, so such an operation should be avoided. Dragonlab's multi-channel pipettes do not have O-rings, and with the tip with a front stop point, a perfect seal can be achieved with just a little pressure, which is really good news for multi-channel pipette users.
A little common sense about the use of pipette tips!
3. Tip immersion angle and depth
The tip immersion angle should be controlled within 20 degrees, and it is better to keep it vertical; the recommended tip immersion depth is as follows: pipette specification tip immersion depth 2µL and 10µL 1 mm 20uL and 100uL 2-3 mm 200uL and 1000uL 3-6 mm5000 µL and 10mL6-10 mm
4. Tip rinsing For samples at room temperature, tip rinsing helps to improve the accuracy; but for high temperature or low temperature samples, tip rinsing reduces the accuracy of operation, please pay special attention to the user.
5. Pipetting speed The pipetting operation should maintain a smooth and appropriate pipetting rate; an excessively fast pipetting rate may easily cause the sample to enter the sleeve, resulting in damage to the piston and sealing ring and cross-contamination of the sample.
