Introduction to the nature, use and production process of serum bottle The serum bottle is made of low-precipitation borosilicate glass, and the colorless transparent bottle meets the requirements of USPI type and ASTME438I standard Chemicalbook standard A. It can be autoclaved at 121℃ for 20min, and can be divided into 125ml, 250ml, 500ml, 1L, 2L according to the volume.
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Serum bottle is a container for storing serum, which is generally made of PET material by injection stretch blowing process. The medium is the nutrient required for cell growth. According to its source, it is divided into synthetic medium and natural medium, both of which can be stored in serum bottles.
The development of cell culture technology has been very mature so far. Cell growth needs to meet various requirements such as environment, temperature, osmotic pressure, pH value, etc. In addition to providing basic nutrients in various synthetic media, it is also necessary to adapt to different cells and different culture purposes. Add serum and other substances to promote cell growth. Therefore, serum bottles have a wide range of applications in the field of cell culture.
Serum is a very complex mixture formed by removing fibrinogen from plasma, mainly including fetal bovine serum, calf serum, adult bovine serum, horse serum, goat serum, chicken serum, rabbit serum, etc. is most commonly used. Serum provides important substances such as extracellular matrix, growth factors and transferrin. The proportion of serum added should be determined according to different cells and different research purposes. 10%~20% serum can maintain the rapid growth and proliferation rate of cells, which is called growth medium; in order to maintain slow growth or immortality of cells, 2%~5% serum can be added, which is called maintenance medium.
Due to the particularity of serum, its storage temperature should be -5°C to -20°C. Serum bottles are generally made of transparent PET material by injection-stretch-blowing technology. This material has good heat aging resistance, embrittlement temperature of -70°C, and still has certain toughness at -30°C, which meets the storage needs of serum. . And the serum bottle will be sterilized, no pyrogen, no endotoxin, no DNase, no RNase and other factors that affect cell growth.
Serum is an indispensable item for cell culture. With the continuous development of biological products, immunotherapy, scientific research institutes and other fields, the demand for serum will also increase, and serum bottles will also be used in the field of cell culture. wider application.
