As a consumable used in conjunction with a pipette, tips can generally be divided into: ①. Standard tips, ②. Filter tips, ③. Low adsorption tips, ④. No heat source tips, etc.
Standard tips are the most widely used and are the most economical category of tips that can be used for almost all pipetting operations.
Filter tip is a consumable designed to avoid cross-contamination, and is often used in molecular biology, cytology, virology and other experiments.
For experiments that require high sensitivity, or precious samples or reagents that are prone to residue, low adsorption tips can be selected to improve recovery. The surface of the low adsorption tip has been hydrophobic treatment, which can reduce the low surface tension liquid leaving more residue in the tip.
PS: Wide mouth pipette, ideal for sucking sticky material, genomic DNA, cell culture medium;
Performance indicators of the tip: low adsorption, filter element, tightness, loading and ejecting force, no DNase and RNase, no pyrogen;
How to choose a suction head? "As long as the tip can fit, it is the tip that can be used"
This is the common perception of almost all users about the adaptability of tips. This statement is partly true but not entirely true.
The tip that can be attached to the pipette can indeed form a pipetting system with the pipette to realize the pipetting function, but is this reliable? A question mark is required here. Answering this question requires data to speak for itself.
1. You may wish to test the performance after matching the pipette with the tip. After rinsing the tip, perform several repeated additions, weigh each addition, and record the reading.
2. Calculate the accuracy and precision of the pipetting operation after converting the density of the test liquid into volume.
3. What we want to choose is a tip with good accuracy. If the matching accuracy of the pipette and tip is not good, it means that the tightness of the tip and the pipette cannot be guaranteed, making the results of each operation unreproducible.
So what should a good suction head have at least?
A good tip depends on concentricity, taper, and most importantly, adsorption;
1. Let’s talk about the taper first: if it is better, the match with the gun will be very good, and the suction will be more accurate;
2. Concentricity: Concentricity refers to whether the circle at the suction nozzle of the suction head and the connection between the suction head and the pipette appear to be the same center of the circle. If it is not the same center, it means that the concentricity is not good;
3. Finally, the most important one is our adsorption: the adsorption is related to the material of the tip. If the material of the tip is not good, it will affect the accuracy of pipetting, resulting in a large amount of liquid retention or short for short. For hanging on the wall, resulting in pipetting errors;
Therefore, everyone should pay special attention to the above three points when choosing a suction head
If a bad tip is placed in a row with obvious spacing, it will make a difference!
