Q: Why don't cells transfer during use?
A: It is very likely that the upper chamber is mixed with a high concentration of serum or the aperture of the small chamber is wrong.
Q: When cells are stained after cell chamber culture, it is found that the distribution of cells is uneven?
A: There may be the following 3 reasons: 1. Whether the chamber is balanced and placed in the well plate 2. Whether there is too much cell suspension when added, the cell suspension should be mixed evenly during use, it is recommended to add dropwise 3. Whether the chamber has Tilt or shake
Q: Why is there a big gap between the multiple holes?
A: The counting is inaccurate or the cells settle when adding multiple chambers. It is recommended to remix after adding a chamber during use.
Q: Why did the cells fail to migrate to the basal compartment in the migration/invasion assay?
A: There may be the following two reasons: 1. The selected membrane pore size is too small. A membrane with a suitable pore size should be selected before use. 2. Air bubbles under the chamber can hinder cell migration, resulting in local no cell migration. You can put it into the chamber first, and then add the medium first to avoid the generation of air bubbles.
Q: In the migration/invasion assay, there is no difference between the control and test groups
A: There may be the following two reasons: 1. The pore size of the membrane used is not suitable, too large or too small will cause cells to migrate easily or difficult to migrate. 2. Insufficient wiping of the upper layer of the chamber membrane. Some experiments only require viewing of cells on the basal side, but cells on both sides will be stained and detected, so wipe off the upper layer.
