Plate streak separation method refers to the use of mixed microorganisms or different cells in the same microbial population with an inoculation loop on the surface of the plate medium by partitioning and streaking to obtain more independent distribution of single cells. A single colony, which is usually regarded as a pure breed of the microorganism to be isolated. Sometimes this single colony is not all propagated from a single cell, so it must be repeatedly isolated many times to obtain a pure breed. The principle is to dilute the microbial samples on the surface of the solid medium for many times "from point to line" to achieve the purpose of separation.
How to use the plate scribing method is as follows: (zanghan infrared sterilizer)
1. Use zanghan brand infrared sterilization, insert the inoculation loop into the sterilizer cavity through the inoculation rod, and sterilize for about 5 seconds
2. Cool next to the infrared sterilizer and open the tampon
3. Pass the test tube mouth through the infrared sterilizer, and use the temperature outside the sterilizer cavity to sterilize the test tube mouth
4. Extend the cooled inoculation loop into the bacterial solution and stick a loop of bacterial solution
5. Pass the mouth of the test tube through the infrared sterilizer again, and quickly cover it with a cotton plug
6. Open the lid of the petri dish with the left hand, stretch the inoculation ring with the bacterial liquid into the plate with the right hand, draw 3-5 parallel lines, cover the vessel, and be careful not to damage the medium
7. Sterilize the inoculation loop again. After it cools, repeat the operation from the end of the first area to the second area, and repeat the line in the third and fourth areas. Be careful not to mix the line in the last area with the first area. connected,
8. Invert the plate and place it in the incubator.
