A cell culture dish is a commonly used glass instrument, a vessel mainly used for microorganism or cell culture, consisting of a flat disc-shaped bottom and a cover, generally made of glass or plastic. Let's introduce its classification and usage precautions.
1. According to the different uses of the culture dish, it can be divided into cell culture dish and bacterial culture dish.
2. According to different manufacturing materials, they are divided into plastic petri dishes and glass petri dishes, but imported petri dishes and disposable petri dishes are mostly made of plastic materials.
3. According to the size, it can usually be divided into diameters of 35mm, 60mm, and 90mm. 150mm petri dish.
4. According to the different partitions, it can be divided into 2-partition petri dishes, 3-partition petri dishes, etc.
5. The materials of cell culture dishes are basically divided into two categories, mainly plastic and glass. Glass can be used for plant materials, microbial culture and animal cell adherent culture. The plastic ones may be made of polyethylene materials, and there are disposable and multiple-use ones, which are suitable for laboratory inoculation, streaking, and isolation of bacteria, and can be used for the cultivation of plant materials.
Operation steps of cell culture dish Keep away from light, store in a dry and cool place, and store in a closed place. It is strictly forbidden to mix and transport with poisonous and harmful items. This product is a non-hazardous product and can be transported as a general chemical, handle it with care, and prevent it from the sun and rain! It is easy to lose vitality after being exposed to heat, moisture and light, and has a short shelf life, so the storage and transportation conditions are relatively harsh.
Instructions for use of cell culture dishes Storage conditions:
Store in a closed, dry and cool place away from light, and it is strictly forbidden to mix and transport with poisonous and harmful items. This product is a non-hazardous product and can be transported as a general chemical, handle it with care, and prevent it from the sun and rain! It is easy to lose vitality after being exposed to heat, moisture and light, and has a short shelf life, so the storage and transportation conditions are relatively harsh.
Precautions for the use of cell culture dishes:
1. After cleaning and disinfection before use, whether the culture dish is clean or not has a great impact on the work, which can affect the pH of the medium. If there are certain chemicals, it will inhibit the growth of bacteria.
2. The newly purchased petri dishes should be rinsed with hot water first, and then soaked in 1% or 2% hydrochloric acid solution for several hours to remove free alkaline substances, and then rinsed twice with distilled water.
3. If you want to cultivate bacteria, use high-pressure steam (generally 6.8*10 to the 5th power of Pa high-pressure steam), sterilize at 120°C for 30 minutes, dry at room temperature, or sterilize with dry heat, that is, put the petri dish Put it in an oven and keep the temperature at about 120°C for 2 hours to kill the bacterial cells.
4. Only sterilized culture dishes can be used for inoculation and culture.
