Lots of questions about PCR products
The thickness of the PCR plates/tubes I bought before is different, is the stability of the thicker ones better?
Of course not. Tube wall thickness directly affects thermal conductivity, and extremely thin wall thicknesses optimize heat transfer and reduce cycle time. The selection of uniform ultra-thin PCR consumables can improve the stable and efficient reaction efficiency of PCR reactions.
So for 96-well or 384-well plates, why do some have skirts and some don't?
In fact, the skirt of the PCR/qPCR plate is to better adapt to automated applications, providing stable support and mechanical strength for the instrument, and higher stability during pipetting.
I don't understand, can you be more specific?
You have too many questions, and my ability is limited to answer them. Let BRAND's product experts answer it for you~
Expert answer 1 - PCR board skirt problem
Generally divided into no skirt, half skirt, full skirt.
Skirtless Plate: Compatible with most PCR machines or qPCR machines, but not suitable for automation, applications. The stability during pipetting is not high and needs to be used with the plate holder.
Half-skirt plate: suitable for labels or barcodes, and automated applications, and has good pipetting stability.
Full Skirt Plate: Ideal for automated laboratory applications, but also suitable for labels and application barcodes. It has good mechanical strength, can be applied to PCR instruments with protruding modules, and has high stability during pipetting.
Expert answer 2 - 96 and 384 well plates are cut corners and marked differently?
A cut corner
The choice of the cut corner position of the PCR plate depends on the requirements of the adapting instrument, which is convenient for positioning.
B logo
Alphanumeric markings on PCR plates help identify individual wells and sample locations.
Generally, it is a protruding color digital logo or an engraved logo. For some automated application experiments, imprinted plates will seal better.
Expert Answer 3 - Why is the edge of the wells of some PCR plates raised?
Common PCR plates have two types of flat wells and raised wells.
The flat-well edge reaction plate is suitable for most PCR machines.
The reaction plate on the edge of the well is easy to seal the membrane, which can reduce the risk of cross-contamination between samples.
Expert Interpretation 4 - There are many types of PCR sealing film, which is better?
According to the specific PCR experimental requirements, the suitable high-quality sealing film is selected, which not only effectively protects the samples and prevents contamination, but also provides reliable experimental test data.
BRAND provides high-quality PCR/qPCR membranes with strong temperature tolerance and sealing, high transparency, and easy handling;
