The principle of nucleic acid detection
This kit is pre-coated with new coronavirus (2019-nCoV) gene recombinant antigen
plate, and with enzyme-labeled anti-human IgA monoclonal antibody as a marker, using ELISA indirect method
To qualitatively detect the novel coronavirus (2019-nCoV) IgA antibody in human serum.
After the reaction complex was added to the substrate TMB, the reaction was terminated, and the O.D. of each well was measured at a wavelength of 450 nm.
The size of the O.D value is proportional to the content of the antibody to be detected.
【Sample requirements】
1. Use fresh blood samples. If you need to save them, you can refrigerate them at 2~8℃ for 48 hours or freeze them at -20℃.
live. Avoid repeated freezing and thawing (more than 3 times).
2. Avoid using soluble, cloudy or fatty specimens.
3. If the specimen needs to be transported, it should be kept at 2~8℃ or frozen.
4. Avoid adding anticoagulants or protective agents or preservatives to the specimen, so as not to affect the test results.
【Inspection method】
1. Take the kit out of the refrigerator and equilibrate to room temperature (15 minutes), remove the pre-coated reaction
should be boarded and unpacked. Dilute the concentrated wash solution 1:30 with distilled or deionized water for use.
2. Specimen dilution: Dilute the specimen to be tested with specimen diluent 1:100 (specimen 2uL---specimen
Diluent 200uL), mix well (or dilute directly in the plate well, that is, add 200ul sample diluent to the plate well, add 2ul sample and mix it).
3. Add sample: Add 100uL of the diluted sample to the well of the plate (if it is diluted in the well of the plate, this step is not required
step). Set a negative and positive control (100uL/well, undiluted) in each well, and set up a blank control well
(Add 100uL specimen diluent), shake and mix.
4. Incubation: 30 minutes at 37°C. Shake off the liquid in the well, wash the plate 3 times with washing solution, each time
After staying for 30 seconds, shake off the liquid in the hole, and pat dry on the absorbent paper.
5. Add enzyme conjugate: 2 drops (or 100uL) per well, mix well and incubate at 37°C for 20 minutes. Wash 3 times as above and pat dry.
6. Color development: add a drop of color developer A and B (or 50uL each) to each well, shake and mix well, set at 37
Incubate for 10 minutes.
