A pipette, also known as a pipette gun, is an instrument for quantitatively transferring liquids and is widely used in biology, chemistry and other fields and in clinical diagnostic laboratories, biotechnology laboratories, pharmacy and chemistry laboratories, environmental laboratories, A common tool in food labs.
pipette pipette
Factors affecting the accuracy of the pipette
(1) Temperature: When the room temperature is low, the hand temperature is high, which makes the air expand, and errors often occur when inhaling the cold solution;
(2) Air tightness: the joint between the tip and the gun body, as well as the long-term wear between the cylinders in the gun, cause errors;
(3) Suction speed: it is easy to cause bubbles in the tip, and it will contaminate the tip;
(4) Reagent volatilization: When sucking a highly volatile reagent, the steam enters the tip of the gun, and the internal pressure increases. As a result, when the liquid is pressed out, the pressure increases, resulting in errors; can be improved.
pipette
Precautions when using
(1) When sucking the liquid, the thumb must be released slowly and steadily, and it is not allowed to release suddenly, in order to prevent the solution from being sucked too fast and rushing into the liquid dispenser to corrode the plunger and cause air leakage;
(2) In order to obtain higher precision, the tip needs to absorb the sample solution once in advance, and then formally pipette, because, when sucking serum protein solution or organic solvent, a layer of "liquid film" will remain on the inner wall of the tip, causing discharge The liquid volume is too small to cause errors;
(3) Liquids with high concentration and viscosity will cause errors. In order to eliminate the compensation amount of the error, it can be determined by experiments. The compensation amount can be set by changing the reading of the reading window with the adjustment knob;
(4) The liquid dispenser can be calibrated by weighing and calculating the weight of the pure water taken with an analytical balance. 1mL of distilled water weighs 0.9982g at 20°C;
(5) It is very inadvisable to tighten the pipette by repeatedly hitting the tip. Long-term operation will loosen the internal parts and damage the pipette;
(6) When the pipette is not equipped with a tip, do not pipette;
(7) When setting the range, please pay attention to rotate to the desired range, the numbers are clearly displayed in the window, and the set range is within the range of the pipette. Do not turn the button out of the range, otherwise, the mechanical device will be stuck and damaged. pipette.
(8) The pipette is strictly prohibited to absorb highly volatile and corrosive liquids (such as concentrated acid, concentrated alkali, organic matter, etc.).
(9) It is strictly forbidden to use a pipette to mix the liquid.
(10) Do not use a large-scale pipette to remove small volumes of liquid, so as not to affect the accuracy. At the same time, if you need to pipette a larger amount of liquid outside the range, please use a pipette for operation.
