As the consumables used in pipettes, tips are generally divided into standard tips, filter tips, low adsorption tips and heating-free tips according to their uses. Standard tips are the most widely used tips. Common tips can be used for almost all pipetting operations, which are the more economical tip types. Filtered tips are a consumable designed to prevent cross contamination. Commonly used in molecular biology, cytology, virology and other experiments. For experiments that require high sensitivity, or precious samples or reagents are prone to residue, low adsorption tips can be selected to improve recovery. The surface of the low adsorption tip is hydrophobic treated to reduce the low surface tension liquid leaving more residue in the tip. Wide-mouth tips are ideal for aspirating sticky substances, genomic DNA, and cell culture fluids.
Tip performance indicators include low retention, filter element, tightness, loading and ejection force, DNase and RNase free, and pyrogen free. So, do you know what a good tip should have at least? A good tip depends on concentricity, taper, and most importantly, suction. Let's talk about taper first. If good, the fit with the gun will be good and the aspiration will be more accurate; concentricity is a straight view of the tip top and where the tip connects to the pipette. Whether it is concentric or not, if it is not concentric, it means that the concentricity is not good; the adsorption is related to the material of the tip, and the poor material of the tip will affect the accuracy of pipetting, resulting in a lot of liquid residue or hanging on the wall for short, resulting in pipetting errors; therefore, When choosing tips, you should pay special attention to the above three points.
How should I choose a filter tip with filter tip? "As long as the tip that can be installed is the tip that can be used", this is the common understanding of almost all users on the adaptability of tips. This statement is arguably partly true but not entirely true. You may wish to perform a performance test after matching the pipette to the tip. After rinsing the tip, repeat the addition several times, weighing each addition and recording the reading. According to the density of the liquid to be measured and converted into volume, the accuracy and precision of the pipetting operation can be calculated. We're going to pick the top ones with good accuracy. If the accuracy of the pipette and the pipette is not good, it means that the tightness of the pipette and the pipette cannot be guaranteed, so that the results of each operation cannot be reproduced. Therefore, pick a good prompt needs data to speak.
