● Put the isolated colony or bacterial liquid in a solid plate, solid slant, liquid test tube or other culture container. Commonly used inoculation tools include inoculation needles, inoculation hooks, inoculation loops, inoculation ears, etc. These inoculation utensils must be sterilized by flame burning. Among them, the inoculation needle and inoculation ear are mostly used for the transfer and puncture culture of bacteria and yeast; the inoculation loop is used for the transfer of bacterial liquid, and the inoculation hook is thick and hard, which is easy to pick up the colonies of actinomycetes and molds, providing 1 μl And 10μl two specifications, sterile packaging, single-use, more convenient and safe.
● The inoculation loop is a commonly used inoculation tool for bacterial culture. It consists of a metal handle like a anatomical needle. The front end is a thin iron wire. The top of the thin iron wire is bent into a ring. Before use, use an alcohol lamp to burn the ring part red. (sterilization), then dipped the strain, spread it evenly on the medium, and finished the inoculation.
● The inoculation loop should be burned before inoculation, its function is to ensure that you will not contaminate the culture during inoculation, and the burning after inoculation is to prevent the bacteria from escaping, contaminating the inoculation room or ultra-clean table, and affecting the overall environment. A measure to reduce pollution rates. Before burning, dip the inoculation needle in some alcohol, and then burn with an alcohol lamp, generally until the front wire turns red three times. Then place the inoculation loop against the medium or the wall of the test tube, or the wall of the conical flask for a while, in order to prevent the temperature from being too high and destroying the bacteria.
● How to use:
1. Scribing method: take the bacteria-containing material and streak the surface of the solid medium.
2. Spot planting method: touch several points on the surface of the solid medium.
3. Pouring method: Put a small amount of bacteria-containing material into a sterile petri dish, pour the melted agar medium at about 48°C, shake well and cool.
4. Puncture method: stick the microorganisms to puncture and enter into the semi-solid medium for deep culture.
5. Invasion and washing method: pick off the bacteria-containing material and rinse in liquid medium.
