1. Turn on the centrifuge switch and press the open button to open the centrifuge cover.
2. According to the requirements of different substances, set the centrifugal time, speed and temperature. There are two types of centrifugal rotation speed (rpm) and centrifugal force (×g/rcf). Generally, at the same rotation speed per minute, the larger the effective radius, the greater the corresponding centrifugal force, g=r×11.18× 10^-6×rpm^2 (where r is the effective centrifugal radius, that is, the length from the centrifuge axis to the bottom of the centrifuge tube).
When low-temperature centrifugation is selected (please use a centrifuge that can cool down), after setting the temperature (for example, 4°C), press the fast temp button on the control panel to start rapid cooling.
3. Weigh the mass of each centrifuge tube. In actual situations, because the samples to be centrifuged usually belong to the same substance, in this case, it is necessary to ensure that the sample volume is the same.
4. Trim.
Generally, the empty space of the centrifuge is an even number.
If the number of tubes to be centrifuged is an even number, symmetrically place the tubes with the same mass in the centrifuge.
If the number of tubes to be centrifuged is odd, there are two ways to place them:
①. Place the even-numbered centrifuge tubes first, and then find a centrifuge tube, make it equal to the mass of the last centrifuge tube, and place it symmetrically.
②. First place
③ centrifuge tubes with the same weight in the centrifuge symmetrically, and place the remaining centrifuge tubes symmetrically.
Close the centrifuge cover and the machine cover, and start the centrifuge. After the centrifugation is complete, carefully take out the sample and cover the centrifuge.
