As the most commonly used manual adjustable precision pipette in liquid operation, some wrong operations during use will bring errors to the operation itself, and some will cause damage to the machine. Here is a short summary:
Error: When assembling the tip, tap the tip repeatedly with the pipette, it is very tight;
Correct: insert the tip, turn left and right to tighten the tip;
Analysis: The impact will cause the internal gear assembly of the pipette to loosen, and long-term operation will affect the accuracy of the pipette;
Error: The tip does not match the pipette, which affects the air tightness; correctly select the tip that matches the pipette and has quality assurance;
Analysis: The mismatch of the tips is mainly manifested in the impact on air tightness. Will directly affect the accuracy of the pipette, usually the pipetting operation will be less than the set amount;
Error: When pipetting, the pipette is tilted to aspirate;
Correct: Inhale vertically;
Analysis: The tilted state causes the pressure surface to change. will affect the pipetting accuracy;
Error: Pipette lays flat on table when tip contains undispensed liquid;
Correct: hang the pipette vertically on the pipette holder;
Analysis: The tip contains undispensed liquid. After the pipette is laid flat, the liquid may flow into the main body of the pipette, affecting the internal parts of the pipette, causing serious damage, poor pollution, etc.;
Mistake: Using a wide-range pipette to remove small amounts of liquid;
Correct: The pipetting volume must be within the range provided by the pipette to meet the inaccurate and inaccurate requirements;
Analysis: Due to the existence of the air cushion, it is the main reason for the inaccuracy of the pipette. The selection of the pipette should be the maximum range, which is closest to the selection principle of the pipetting volume;
Error: Absorb highly volatile liquids;
Correct: It is not recommended to use ordinary pipettes for strongly volatile liquid transfer;
Analysis: If highly volatile liquids must be removed, the pipette should be disassembled immediately after pipetting to allow the vapors to evaporate. At the same time, it is recommended to use an external piston pipette;
Error: The aspiration speed and dispensing speed are too fast;
Correct: breathe slowly and release slowly;
Analysis: Quick Aspiration For pipettes with a volume of 1mL and above, it is easy to cause liquid overshoot. Unloading too quickly will increase the liquid residue.
